PDHB antibody (AA 31-341)

Catalog No. ABIN1860165

Product Details anti-PDHB Antibody

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Binding Specificity: AA 31-341

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDHb. It has been selected for its ability to recognize PDHb in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Pyruvate Dehydrogenase Beta (PDHb) corresdonding to Leu31~Asn341 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: Pyruvate Dehydrogenase Beta (PDHB Products)

Synonyms: CG 11876 antibody, Dmel\\CG11876 antibody, E1beta antibody, PDHB antibody, zgc:64062 antibody, wu:fc76a05 antibody, PDHBD antibody, PDHE1-B antibody, PHE1B antibody, 2610103L06Rik antibody, AL024199 antibody, C81408 antibody, CG11876 gene product from transcript CG11876-RA antibody, pyruvate dehydrogenase E1 beta subunit antibody, pyruvate dehydrogenase (lipoamide) beta antibody, CG11876 antibody, pdhb antibody, PDHB antibody, Pdhb antibody

Background: PDH-B, PHE1B, PHE1-B, Pyruvate Dehydrogenase(lipoamide)Beta

Pathways: Warburg Effect