EIF2AK2 antibody (AA 279-506)

Catalog No. ABIN1860247

This Rabbit Polyclonal antibody specifically detects EIF2AK2 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for EIF2AK2 antibody (AA 279-506) (ABIN1860247)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-EIF2AK2 Antibody

Binding Specificity: AA 279-506

Purpose: Polyclonal Antibody to Protein Kinase R (PKR)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKR. It has been selected for its ability to recognize PKR in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA520Ra01Recombinant Protein Kinase R (PKR)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: Protein Kinase R

Background: PRKR, PRK-R, EIF2AK2, EIF2AK1, Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, P1/eIF-2A kinase

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C