PPARG antibody (AA 311-500)

Catalog No. ABIN1860282

Cited in 1 publication. The Rabbit Polyclonal anti-PPARG antibody (ABIN1860282) specifically detects PPARG in WB, IHC, IP and ICC. The antibody is reactive with Cow samples.

Quick Overview for PPARG antibody (AA 311-500) (ABIN1860282)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PPARG Antibody

Binding Specificity: AA 311-500

Purpose: Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)

Specificity: The antibody is a rabbit polyclonal antibody raised against PPARg. It has been selected for its ability to recognize PPARg in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mLImmunohistochemistry: 5-20 μg/mLImmunocytochemistry: 5-20 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-PPARG antibody (ABIN1860282)

Montanari, Colitti: "Simpson-Golabi-Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiation." in: Histochemistry and cell biology, Vol. 149, Issue 6, pp. 593-605, (2018) (PubMed).

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARg

Background: PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation