IFNA antibody (AA 24-172) (Biotin)

Catalog No. ABIN1862653

Product Details anti-IFNA Antibody (Biotin)

Target: IFNA (Interferon alpha (IFNA))

Binding Specificity: AA 24-172

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - CDLPQTH SLAHTRALRL LAQMRRISPF SCLDHRRDFG SPHEAFGGNQ VQKAQAMALV HEMLQQTFQL FSTEGSAAAW NESLLHQFYT GLDQQLRDLE ACVMQEAGLE GTPLLEEDSI RAVRKYFHRL TLYLQEKSYS PCAWEIVRAE VM

Specificity: It has been selected for its ability to recognize IFNa in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant IFNa expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against IFNa conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant IFNa (Cys24~Met172) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for IFNA

Target: IFNA (Interferon alpha (IFNA))

Alternative Name: Interferon Alpha (IFNA Products)

Synonyms: IFN-alphaO antibody, IFN-ALPHA-1 antibody, IFN1@ antibody, Ifa antibody, Ifa8 antibody, interferon alpha 16 antibody, interferon, alpha 1 antibody, interferon alpha antibody, interferon-alpha antibody, IFNA16 antibody, IFNA1 antibody, Ifna antibody, IFNA antibody

Pathways: JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome