Sclerostin antibody (AA 25-213)
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- Target See all Sclerostin (SOST) Antibodies
- Sclerostin (SOST)
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Binding Specificity
- AA 25-213
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Sclerostin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Sclerostin (SOST)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against SOST. It has been selected for its ability to recognize SOST in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Pig
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Sclerostin (SOST) corresdonding to Gly25~Tyr213 with N-terminal His and GST Tag
- Isotype
- IgG
- Top Product
- Discover our top product SOST Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:150-600 Immunohistochemistry: 5-20 μg/mL
1:15-60 Immunocytochemistry: 5-20 μg/mL
1:15-60 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Sclerostin (SOST)
- Alternative Name
- Sclerostin (SOST Products)
- Synonyms
- LOC100313724 antibody, CDD antibody, VBCH antibody, 5430411E23Rik antibody, sclerostin antibody, LOC100313724 antibody, SOST antibody, Sost antibody
- Background
- VBCH, Sclerosteosis
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