ALIX antibody (AA 541-570)

Catalog No. ABIN1881644

There are 4+ publications for this product available. The Rabbit Polyclonal anti-ALIX antibody is suitable to detect ALIX in samples from Human. It has been validated for WB.

Quick Overview for ALIX antibody (AA 541-570) (ABIN1881644)

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALIX antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB41924

Product Details anti-ALIX Antibody

Binding Specificity: AA 541-570

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PDCD6IP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-570 amino acids from the Central region of human PDCD6IP.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ALIX antibody (ABIN1881644)

Inuzuka, Suzuki, Kawasaki, Shibata, Wakatsuki, Maki: "Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition." in: BMC structural biology, Vol. 10, pp. 25, (2010) (PubMed).

Shi, Opi, Lugari, Restouin, Coursindel, Parrot, Perez, Madore, Zimmermann, Corbeil, Huang, Arold, Collette, Morelli: "Identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X." in: The Biochemical journal, Vol. 431, Issue 1, pp. 93-102, (2010) (PubMed).

Irie, Inoue, Sakaguchi: "Significance of the YLDL motif in the M protein and Alix/AIP1 for Sendai virus budding in the context of virus infection." in: Virology, Vol. 405, Issue 2, pp. 334-41, (2010) (PubMed).

Sette, Jadwin, Dussupt, Bello, Bouamr: "The ESCRT-associated protein Alix recruits the ubiquitin ligase Nedd4-1 to facilitate HIV-1 release through the LYPXnL L domain motif." in: Journal of virology, Vol. 84, Issue 16, pp. 8181-92, (2010) (PubMed).

Target Details for ALIX

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Alternative Name: PDCD6IP

Background: This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Molecular Weight: 96023

NCBI Accession: NP_001155901, NP_037506

UniProt: Q8WUM4

Pathways: p53 Signaling, EGFR Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Tube Formation