Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This POLDIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human POLDIP3.
POLDIP3
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
IF: 1:25. WB: 1:1000. IHC-P: 1:25
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Ma, Yoon, Richardson, Jülich, Blenis: "SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs." in: Cell, Vol. 133, Issue 2, pp. 303-13, (2008) (PubMed).
Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, (2007) (PubMed).
Smyk, Szuminska, Uniewicz, Graves, Kozlowski: "Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase delta and S6K1 and regulating cell growth." in: The FEBS journal, Vol. 273, Issue 20, pp. 4728-41, (2006) (PubMed).
Richardson, Bröenstrup, Fingar, Jülich, Ballif, Gygi, Blenis: "SKAR is a specific target of S6 kinase 1 in cell growth control." in: Current biology : CB, Vol. 14, Issue 17, pp. 1540-9, (2004) (PubMed).
Target
POLDIP3
(Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))
PDIP46 antibody, SKAR antibody, 1110008P04Rik antibody, AA408269 antibody, AL022852 antibody, C77954 antibody, mKIAA1649 antibody, polymerase (DNA-directed), delta interacting protein 3 S homeolog antibody, DNA polymerase delta interacting protein 3 antibody, polymerase (DNA-directed), delta interacting protein 3 antibody, poldip3.S antibody, POLDIP3 antibody, Poldip3 antibody
Background
This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified. [provided by RefSeq].