PCNA antibody (C-Term)

Catalog No. ABIN1882273

This Mouse Monoclonal antibody specifically detects PCNA in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for PCNA antibody (C-Term) (ABIN1882273)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1157CT1-1-3

Product Details anti-PCNA Antibody

Binding Specificity: AA 236-261, C-Term

Predicted Reactivity: B, Ha, Pr

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PCNA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 236-261 amino acids from the C-terminal region of human PCNA.

Isotype: IgG1

Application Details

Application Notes: WB: 1:2000. IHC: 1:2000. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PCNA antibody (ABIN1882273)

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Molecular Weight: 28769

NCBI Accession: NP_002583, NP_872590

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy