PFKM antibody (AA 1-223)

Catalog No. ABIN1885803

The Rabbit Polyclonal anti-PFKM antibody (ABIN1885803) specifically detects PFKM in WB. The antibody is reactive with Human samples.

Quick Overview for PFKM antibody (AA 1-223) (ABIN1885803)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFKM Antibody

Binding Specificity: AA 1-223

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 223 of Human PFKM

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFK-1

Background: The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis.Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL, MIM 171860), and platelet (PFKP, MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively.The composition of the tetramers differs according to the tissue type.Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively.Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721], Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]

Molecular Weight: 85 kDa

Gene ID: 5213

NCBI Accession: NM_000289, NP_000280

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect