Caspase 8 antibody (AA 1-227)

Catalog No. ABIN1885811

The Rabbit Polyclonal anti-Caspase 8 antibody has been validated for WB, IHC and IF. It is suitable to detect Caspase 8 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 8 antibody (AA 1-227) (ABIN1885811)

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 1-227

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 227 of Human CASP8

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.100-1.250
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: caspase-8

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family.Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit.Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits.This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli.The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD.This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases.Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.[provided by RefSeq]

Molecular Weight: 58 kDa

Gene ID: 841

NCBI Accession: NP_001219, NM_001228

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades