Kininogen LC (AA 1-406) antibody
Reactivity: Human
IF, IHC, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- Kininogen LC
- Binding Specificity
- AA 1-406
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 406 of Human KNG1
-
- Application Notes
-
Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.50-1.500
Immunofluorescence: 1.100-1.200 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 0.1 M Tris-buffered saline with 20 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- Biohazard Informations: This product contains thimerosal which is hazardous.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
-
- Target
- Kininogen LC
- Background
-
High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein (see MIM 147910)-kinin system.The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing.Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3.However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L.In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide.BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin (see MIM 601699) from endothelial cells.During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy.Release of BK from HMWK generates a 2-chain HMWK, termed HMWKa, containing the heavy and light chains joined by a disulfide bond (Merkulov et al., 2008 [PubMed 18000168]).[supplied by OMIM]
- Molecular Weight
- 48 kDa
- Gene ID
- 3827
- NCBI Accession
- NP_000884, NM_000893
-
You are here:
