GEMC1 antibody (Center)

Catalog No. ABIN1887716

Product Details anti-GEMC1 Antibody

Target: GEMC1 (GMNC) (Geminin Coiled-Coil Domain Containing (GMNC))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GEMC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Affinity chromatography purified via peptide column

Immunogen: 19 amino acid peptide near the center of human GEMC1.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freezing and thawing repeatly.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term use.Store at -20 °C for long term preservation.

Target Details for GEMC1

Target: GEMC1 (GMNC) (Geminin Coiled-Coil Domain Containing (GMNC))

Alternative Name: GEMC1 (GMNC Products)

Synonyms: GEMC1 antibody, Gemc1 antibody, Gm1778 antibody, Gm606 antibody, gemc1 antibody, xGEMC1 antibody, geminin coiled-coil domain containing antibody, geminin coiled-coil domain containing S homeolog antibody, GMNC antibody, Gmnc antibody, gmnc.S antibody

Background: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication.GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function.GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation.GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication.Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.
Synonyms: Geminin coiled-coil domain-containing protein 1

NCBI Accession: NP_001140158