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Geminin Coiled-Coil Domain Containing (GMNC) (Center) antibody Primary Antibody

GMNC Reactivity: Human, Mouse ELISA, WB Host: Rabbit Polyclonal
Catalog No. ABIN1887716
$581.90
Plus shipping costs $45.00
100 μL
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  • Target
    Binding Specificity
    Center
    Reactivity
    Human, Mouse
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    ELISA, Western Blotting (WB)
    Purification
    Affinity chromatography purified via peptide column
    Immunogen
    19 amino acid peptide near the center of human GEMC1.
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid freezing and thawing repeatly.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4 °C for short term use.Store at -20 °C for long term preservation.
  • Target
    Alternative Name
    GEMC1 (GMNC Antibody Abstract)
    Background
    The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication.GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function.GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation.GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication.Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.
    Synonyms: Geminin coiled-coil domain-containing protein 1
    NCBI Accession
    NP_001140158
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