PCNA antibody (AA 75-125)

Catalog No. ABIN1922194

This Rabbit Polyclonal antibody specifically detects PCNA in WB, IHC, IF, FACS, IHC (p), IP and PLA. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Dog, Rabbit, Cow and Horse.

Quick Overview for PCNA antibody (AA 75-125) (ABIN1922194)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Dog, Rabbit, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Product Details anti-PCNA Antibody

Binding Specificity: AA 75-125

Specificity: Region between residues 75 and 125 of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: Flo, IF (1:1000 - 1:5000), IHC, IHC-P (1:2000 - 1:10000), IP (2 - 5 μg/mg lysate), PLA (1:1000 - 1:10000), WB (1:1000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma .

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA / Cyclin

Background: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

Gene ID: 5111

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy