GABA B Receptor antibody

Catalog No. ABIN1944944

Product Details

Target: GABA B Receptor

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Application Details

Application Notes: WB: 1:1000. IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References

Kaupmann, Schuler, Mosbacher, Bischoff, Bittiger, Heid, Froestl, Leonhard, Pfaff, Karschin, Bettler: "Human gamma-aminobutyric acid type B receptors are differentially expressed and regulate inwardly rectifying K+ channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 25, pp. 14991-6, (1999) (PubMed).

Goei, Choi, Ahn, Bowlus, Raha-Chowdhury, Gruen: "Human gamma-aminobutyric acid B receptor gene: complementary DNA cloning, expression, chromosomal location, and genomic organization." in: Biological psychiatry, Vol. 44, Issue 8, pp. 659-66, (1999) (PubMed).

White, Wise, Main, Green, Fraser, Disney, Barnes, Emson, Foord, Marshall: "Heterodimerization is required for the formation of a functional GABA(B) receptor." in: Nature, Vol. 396, Issue 6712, pp. 679-82, (1999) (PubMed).

Grifa, Totaro, Rommens, Carella, Roetto, Borgato, Zelante, Gasparini: "GABA (gamma-amino-butyric acid) neurotransmission: identification and fine mapping of the human GABAB receptor gene." in: Biochemical and biophysical research communications, Vol. 250, Issue 2, pp. 240-5, (1998) (PubMed).

Target Details

Target: GABA B Receptor

Alternative Name: GABA-B Receptor

Background: Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.

Molecular Weight: 108320 Da

Gene ID: 2550

UniProt: Q9UBS5