Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
Specificity
This JARID2 antibody is useful for Immunocytochemistry/Immunofluorescence (staining is nuclear) and Western blot. By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. This antibody has also been reported to work in Chromatin Immunoprecipitation (ChIP), see PMID: 22396653.
Purification
Aff - Purified
Immunogen
A synthetic peptide made to an N-terminal portion of the human JARID2 or jumonji protein sequence (AA 1-100).
JARID2
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200 , Western Blot 2µg/mL, Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.83 mg/mL
Buffer
Tris-glycine 150mM NaCl with 0.05% Sodium Azide as a preservative.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Repeated freeze/thaw cycles
Storage
-20 °C
Storage Comment
Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C. Compromise the integrity of the antiserum.
Target
JARID2
(Jumonji, AT Rich Interactive Domain 2 (JARID2))