TNF alpha antibody

Catalog No. ABIN1959983

The Mouse Monoclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Human, Pig and Primate. It has been validated for IHC, ELISA, FACS, ICC, Neut, Func, IHC (fro) and BR.

Quick Overview for TNF alpha antibody (ABIN1959983)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Pig, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Clone: MAb11

Product Details anti-TNF alpha Antibody

Specificity: Monoclonal antibody MAb11 recognizes human 17-26 kD cytokine TNF-alpha (tumor necrosis factor alpha).

Purification: Protein A purified

Immunogen: Recombinant human TNF-alpha.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: Block, ELISA, Flo, Func, ICC, IHC, IHC-Fr, Neut

Usage: Flow Cytometry: Application note - intracellular staining. Immunohistochemistry (frozen sections): Application note - paraformaldehyde-fixed, saponin-treated frozen tissue sections. ELISA: Biotinylated MAb11 can be used as a detection antibody in combinat

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome