GFAP antibody

Catalog No. ABIN1967166

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Human GFAP

Purification: Purified

Immunogen: The initial challenge was performed with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with GFAP purified from a Triton X-100 extract of myelin associated material from bovine spinal cord, following an axonal flotation procedure. The GFAP was further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose.

Type of Immunogen: Recombinant protein

Isotype: IgY

Application Details

Application Notes: Approved: ICC (1:1000), IHC (1:1000), WB (1:100000)

Usage: For immunocytochemistry on cells in tissue culture or in tissue sections, try this antibody at about between about 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme linked methods. For immunoblotting 1:50000 is recommended. Expect to see a band at 55  kDa and another at about 48  kDa, apparently a breakdown product of the 55  kDa band.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

Background: Name/Gene ID: GFAP

Synonyms: GFAP

Gene ID: 2670