ATM antibody (pSer1981)

Catalog No. ABIN196822

This Rabbit Polyclonal antibody specifically detects ATM in IHC (p). It exhibits reactivity toward Human.

Quick Overview for ATM antibody (pSer1981) (ABIN196822)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATM antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATM Antibody

Binding Specificity: pSer1981

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. ATM (phospho-Ser1981) antibody detects endogenous levels of ATM only when phosphorylated at serine 1981.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ATM around the phosphorylation site of serine 1981 (E-G-SP-Q-S).

Application Details

Application Notes: Suitable for use in Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).Synonyms: A-T mutated, ATDC, Ataxia telangiectasia mutated, Serine-protein kinase ATM, TEL1, TELO1

Gene ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus