PAK1 antibody (pThr212)

Catalog No. ABIN196878

Product Details anti-PAK1 Antibody

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Binding Specificity: pThr212

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody detects endogenous levels of PAK1 only when phosphorylated at Threonine 212.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human PAK1 around the phosphorylation site of threonine 212 (P-V-Tp-P-T).

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1 (PAK1 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, PAKalpha antibody, AW045634 antibody, PAK-1 antibody, Paka antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, p21 protein (Cdc42/Rac)-activated kinase 1 antibody, Serine/threonine-protein kinase pak-1 antibody, pak1.S antibody, PAK1 antibody, Pak1 antibody, pak-1 antibody

Background: The serine/threonine kinase Pak1 is an effector of small Rho GTPases, Rac1 and Cdc42. Pak1 complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. It plays an important role in the regulation of cell morphogenesis, motility, mitosis, and angiogenesis and has been implicated in the progression of many cancers.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK

Gene ID: 5058

NCBI Accession: NP_001122092

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis