Bcl-2 antibody (AA 50-100)

Catalog No. ABIN1976984

The Rabbit Polyclonal anti-Bcl-2 antibody is suitable to detect Bcl-2 in samples from Human. It has been validated for IHC, IF, IP and IHC (p).

Quick Overview for Bcl-2 antibody (AA 50-100) (ABIN1976984)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 50-100

Specificity: Region between residue 50 and 100 of human B-cell CLL/lymphoma 2 using the numbering given in entry NP_000624.2 (GeneID 596).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:1000), IHC, IHC-P (1:500 - 1:2000), IP (2 - 10 μg/mg lysate)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Colon Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Prostate Carcinoma.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL2 / Bcl-2

Background: Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50

Gene ID: 596

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling