ENO1 antibody

Catalog No. ABIN1978808

Product Details anti-ENO1 Antibody

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human, Mouse, Rat, Cow, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Clone 253 is known to react with enolase 1 from human, bovine, mouse, rat and Xenopus. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1.

Purification: Affinity purified

Immunogen: N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system.

Clone: 253

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ICC (1:2000 - 1:5000), IF (1:2000 - 1:5000), WB (1:5000 - 1:10000)

Usage: Try at dilutions of 1:2000-1:5000 for immunofluorescence. For western blots try at 1:5000-1:10000. The NNE protein runs at about ~47 kDa on SDS-PAGE gels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 10 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1 / alpha Enolase (ENO1 Products)

Synonyms: enolase 1 antibody, F2P9.10 antibody, F2P9_10 antibody, Pgh1 antibody, pENO1 antibody, enolase 1 antibody, phosphopyruvate hydratase antibody, ENO1 antibody, eno1 antibody

Background: Name/Gene ID: ENO1

Synonyms: ENO1, Alpha Enolase, Alpha enolase like 1, ENO1L1, Enolase 1, MBP-1, MBPB1, MPB1, MYC promoter-binding protein 1, NNE, Phosphopyruvate hydratase, Alpha-enolase, Tau-crystallin, MBP1, MPB-1, C-myc promoter-binding protein, Enolase 1, (alpha), Enolase-alpha, Non-neural enolase, Plasminogen-binding protein, PPH

Gene ID: 2023