STAT5A antibody (C-Term)

Catalog No. ABIN198072

The Mouse Monoclonal anti-STAT5A antibody (ABIN198072) specifically detects STAT5A in WB, ELISA, IP and EMSA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for STAT5A antibody (C-Term) (ABIN198072)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA)

Product Details anti-STAT5A Antibody

Binding Specificity: C-Term

Specificity: Reacts specifically with the STAT5a protein. Does not cross-react with the highly related STAT5b protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by Western Blot using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells. Species cross-reactivity: Human, mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to the C-terminus of murine Stat5a (carrier protein coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), GS, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 5 μg. Western Blot: 1-3 μg/mL. Gel Mobility Shift Assay: 1-3 μg.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A

Background: Name/Gene ID: STAT5A

Synonyms: STAT5A, MGF, STAT5

Gene ID: 6776

UniProt: P42229

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation