STAT5B antibody (C-Term)

Catalog No. ABIN198076

This Mouse Monoclonal antibody specifically detects STAT5B in WB, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for STAT5B antibody (C-Term) (ABIN198076)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-STAT5B Antibody

Binding Specificity: C-Term

Specificity: Reacts specifically with the STAT 5b protein and does not exhibit any cross-reactivity with the highly related STAT 5a protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Species cross-reactivity: Human, mouse and rat. Reactivity of this antibody with STAT5b has been confirmed by Western blotting using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells.

Purification: Immunoaffinity purified

Immunogen: A peptide corresponding to the unique carboxy-terminus of murine STAT5b coupled to a protein carrier.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a lambda

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Electrophoretic Mobility Shift Assay (EMSA). ELISA: 0.1-1 μg/mL. Immunoprecipitation: 5 μg. Western Blot: 1-3 μg/mL. Electrophoretic Mobility Shift Assay (EMSA): 1-3 μg.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Alternative Name: STAT5B / STAT

Background: Name/Gene ID: STAT5B

Synonyms: STAT5B, Transcription factor STAT5B, STAT5

Gene ID: 6777

UniProt: P51692

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation