Integrin beta 4 antibody

Catalog No. ABIN198907

Product Details anti-Integrin beta 4 Antibody

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: Recognizes human b4 integrin (CD104).

Purification: Protein A purified

Immunogen: Human squamous cell carcinoma cells (SCC9).

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo, Func, IHC, IHC-Fr, IP

Usage: Suitable for use in Inhibition of Cell Attachment, Immunohistochemistry, Flow Cytometry and Immunoprecipitation. Inhibition of cell attachment: Inhibits SCC9 cell adhesion to human laminin and laminin GD-2 peptide, but not to EHS laminin, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen. Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets. Immunoprecipitation: Precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: ITGB4 / Integrin Beta 4 (ITGB4 Products)

Synonyms: AA407042 antibody, C230078O20 antibody, CD104 antibody, integrin subunit beta 4 antibody, integrin beta 4 antibody, ITGB4 antibody, Itgb4 antibody

Background: Name/Gene ID: ITGB4
Family: Integrin

Synonyms: ITGB4, CD104, Integrin beta-4, Integrin Beta 4, gp150, CD104 antigen, Integrin beta-4 subunit, Integrin, beta 4

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex