BUB1 antibody (AA 281-419)

Catalog No. ABIN199933

Product Details anti-BUB1 Antibody

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Binding Specificity: AA 281-419

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: Amino acid residues 281-419 of human BUB1 protein.

Purification: Protein A purified

Immunogen: Recombinant human Bub1.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: IF, IP, WB

Usage: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~160 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at  pH 6.8 OR 100 % methanol at -20? C. Permeabilize fixed cells with 0.5 % Triton X-100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1 (BUB1 Products)

Synonyms: bub1a antibody, bub1l antibody, hbub1 antibody, xbub1 antibody, BUB1A antibody, BUB1L antibody, hBUB1 antibody, AL022991 antibody, Bub1a antibody, C80208 antibody, D2Xrf87 antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, mitotic checkpoint serine/threonine kinase antibody, BUB1 antibody, bub1 antibody, Bub1 antibody

Background: Name/Gene ID: BUB1
Subfamily: BUB1
Family: Protein Kinase

Synonyms: BUB1, Bub1A, BUB1L, HBUB1

Gene ID: 699

UniProt: O43683