His Tag antibody (iFluor 555)

Catalog No. ABIN2017573

This Mouse Monoclonal antibody specifically detects His Tag in IF.

Quick Overview for His Tag antibody (iFluor 555) (ABIN2017573)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to iFluor 555

Application: Immunofluorescence (IF)

Clone: 6G2A9

Product Details anti-His Tag Antibody (iFluor 555)

Sequence: HHHHHH

Characteristics: THETM His Tag Antibody [iFluor 555], mAb, Mouse is THETM His Tag Antibody (A00186) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.

Purification: Protein A affinity column

Immunogen: A synthetic peptide HHHHHH coupled to KLH

Isotype: IgG1 kappa

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining:1-4 μg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.