Angiotensin I Converting Enzyme 1 antibody (N-Term)

Catalog No. ABIN204334

This Mouse Monoclonal antibody specifically detects Angiotensin I Converting Enzyme 1 in WB, IHC, ELISA, IP, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Monkey, Cat and Hamster.

Quick Overview for Angiotensin I Converting Enzyme 1 antibody (N-Term) (ABIN204334)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Human, Rat, Monkey, Cat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Angiotensin I Converting Enzyme 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Angiotensin I Converting Enzyme 1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes N-terminal epitope of Angiotensin-converting enzyme (ACE). Species cross-reactivity: Human, monkey, rat, hamster and feline.

Purification: Purified

Immunogen: Native angiotensin-converting enzyme from human lung.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:1000), ICC (1:100), IHC, IHC-Fr, IHC-P, IP (1:1000 - 1:10000), WB (1:100)

Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and ELISA. Immunohistochemistry: 1:1000 on fresh frozen or 4 % paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue. Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line). Western Blot: 1:100. Immunoprecipitation: 1:1000-1:10000. ELISA: 1:1000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE / DCP1 / CD143

Background: Name/Gene ID: ACE
Subfamily: Metallopeptidase M2
Family: Protease

Synonyms: ACE, ACE1, Angiotensin-converting enzyme, CD143, CD143 antigen, Carboxycathepsin, DIPEPTIDYL CARBOXYPEPTIDASE, DCP, Dipeptidyl carboxypeptidase I, ICH, Kininase II, MVCD3, Peptidyl-dipeptidase A, Peptidase P, DCP1, Dipeptidyl carboxypeptidase 1, Testicular ECA

Gene ID: 1636

UniProt: P12821

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration