NGFR antibody (AA 274-425)

Catalog No. ABIN204431

Product Details anti-NGFR Antibody

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Binding Specificity: AA 274-425

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: Recognizes p75 neurotrophin receptor, Mr ~75kD. Species cross-reactivity: Rat, human and mouse.

Purification: Protein A purified

Immunogen: GST fusion protein corresponding to the intracellular domain (residues 274-425) of rat p75 neurotrophin receptor.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP, WB (0.1 - 5 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.1-05 μg/mL detected p75 neurotrophin receptor in RIPA lysates from PC-12 cells. PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p75 Neurotrophin Receptor (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate p75 neurotrophin receptor. Immunohistochemistry: Reported to detect p75 neurotrophin receptor in rat brain sections.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR / CD271 / p75 (NGFR Products)

Synonyms: ngfr antibody, wu:fb38c02 antibody, wu:fc10h01 antibody, CD271 antibody, Gp80-LNGFR antibody, TNFRSF16 antibody, p75(NTR) antibody, p75NTR antibody, LNGFR antibody, RNNGFRR antibody, Tnfrsf16 antibody, p75 antibody, NRH1b antibody, fullback antibody, nrh1 antibody, p75ntr antibody, tnfrsf16 antibody, p75NGFR antibody, nerve growth factor receptor antibody, neurotrophin receptor associated death domain antibody, nerve growth factor receptor S homeolog antibody, nerve growth factor receptor (TNFR superfamily, member 16) antibody, NGFR antibody, nradd antibody, Ngfr antibody, ngfr.S antibody

Background: Name/Gene ID: NGFR
Family: TNF Receptor

Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding