GZMB antibody

Catalog No. ABIN206367

The Mouse Monoclonal anti-GZMB antibody (Clone GB11) (ABIN206367) specifically detects GZMB in IHC, ELISA, FACS, IP and IHC (fro). The antibody is reactive with Human, Monkey and Rhesus Monkey samples.

Quick Overview for GZMB antibody (ABIN206367)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human, Monkey, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: GB11

Product Details anti-GZMB Antibody

Specificity: Recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death. Has been reported to function as a capture reagent in ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (MCA2119B) as detection reagent.

Purification: Protein A purified

Immunogen: Purified human Granzyme B.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 5 μg/mL), Flo (1:10 - 1:100), IHC, IHC-Fr, IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. ELISA: This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (LS-C44294) as detection reagent.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris buffered saline, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: GZMB / Granzyme B

Background: Name/Gene ID: GZMB
Subfamily: Serine S1
Family: Protease

Synonyms: GZMB, CCPI, CGL-1, Cathepsin G-like 1, CSP-B, CTLA-1, CTLA1, CGL1, CSPB, Fragmentin 2, GRB, HLP, Human lymphocyte protein, Fragmentin-2, SECT, Lymphocyte protease, T-cell serine protease 1-3E, C11, CTSGL1, Cytotoxic serine protease B, Granzyme B, Granzyme-2

Gene ID: 3002

Pathways: Apoptosis, Caspase Cascade in Apoptosis