SERPINB9 antibody
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- Target See all SERPINB9 Antibodies
- SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SERPINB9 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- Specificity
- Specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone Detects a band of approximately 42kD under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence.
- No Cross-Reactivity
- Mouse (Murine), Pig (Porcine)
- Purification
- Protein G purified
- Immunogen
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Recombinant PI-9 produced in P. pastoris.
Type of Immunogen: Recombinant protein - Isotype
- IgG1
- Top Product
- Discover our top product SERPINB9 Primary Antibody
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- Application Notes
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Approved: Flo (1:10 - 1:100), IHC, IHC-P (1:50), WB
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Tonsil. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))
- Alternative Name
- SERPINB9 / PI9 (SERPINB9 Products)
- Synonyms
- SERPINB9 antibody, BB283241 antibody, CAP-3 antibody, CAP3 antibody, PI-9 antibody, PI9 antibody, Spi6 antibody, ovalbumin antibody, serpin family B member 9 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 9 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 9 antibody, Serpinb9 antibody, SERPINB9 antibody
- Background
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Name/Gene ID: SERPINB9
Synonyms: SERPINB9, CAP-3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, Serpin B9, PI-9, PI9 - Gene ID
- 5272
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