MMP14 antibody (AA 319-333)
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- Target See all MMP14 Antibodies
- MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
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Binding Specificity
- AA 319-333
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Reactivity
- Human, Mouse, Rat, Cow, Monkey, Goat, Hamster, Sheep
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MMP14 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).
- Purification
- Immunoaffinity purified
- Immunogen
- Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).
- Isotype
- IgG3 kappa
- Top Product
- Discover our top product MMP14 Primary Antibody
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- Application Notes
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Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.0, 2 % BSA (protease free).
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
- Alternative Name
- MMP14 / MMP-14 (MMP14 Products)
- Synonyms
- MMP1 antibody, MMP-14 antibody, MMP-X1 antibody, MT-MMP antibody, MT-MMP 1 antibody, MT1-MMP antibody, MT1MMP antibody, MTMMP1 antibody, mmp-x1 antibody, mt1-mmp antibody, mtmmp1 antibody, AI325305 antibody, MT-MMP-1 antibody, sabe antibody, Mt1-mmp antibody, MT-MMPbeta antibody, cb386 antibody, sb:cb386 antibody, wu:fb72b09 antibody, mmp14 antibody, matrix metallopeptidase 14 antibody, matrix metallopeptidase 14 (membrane-inserted) antibody, matrix metallopeptidase 14b (membrane-inserted) antibody, matrix metallopeptidase 14a (membrane-inserted) antibody, matrix metallopeptidase 14 L homeolog antibody, MMP14 antibody, mmp14 antibody, Mmp14 antibody, mmp14b antibody, mmp14a antibody, mmp14.L antibody
- Background
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Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease
Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1 - Gene ID
- 4323
- UniProt
- P50281
- Pathways
- Autophagy
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