PCNA antibody (Biotin)

Catalog No. ABIN209149

This Mouse Monoclonal antibody specifically detects PCNA in WB, IHC, IF, IHC (p) and ICC. It exhibits reactivity toward Human, Mouse, Rat, Chicken and Xenopus laevis.

Quick Overview for PCNA antibody (Biotin) (ABIN209149)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-PCNA Antibody (Biotin)

Specificity: Recognizes human PCNA. Reacts with all vertebrate species, insects and S. pombe on Western blots. Species cross-reactivity: mouse, rat, chicken, xenopus.

Purification: Purified

Immunogen: Recombinant PCNA polypeptides.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-P (1:5 - 1:20), WB (1:500 - 1:2000)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 1:500-1:2000. Immunohistochemistry: 1:5-1:20 (Alcohol or formalin-fixed, paraffin-embedded). Positive control: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol and NIH 3T3 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 0.1 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA / Cyclin

Background: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy