Integrin beta 2 antibody (Activation Site)

Catalog No. ABIN210134

The Mouse Monoclonal anti-Integrin beta 2 antibody is suitable to detect Integrin beta 2 in samples from Human. It has been validated for FACS, WB and IP.

Quick Overview for Integrin beta 2 antibody (Activation Site) (ABIN210134)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: MEM-148

Product Details anti-Integrin beta 2 Antibody

Binding Specificity: Activation Site

Specificity: Recognizes an epitope on the human CD18 Molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by MEM-148 is also exposed during dissociation of the CD11/CD18 by low  pH .

Purification: Protein G purified

Immunogen: Peripheral blood mononuclear cells. (ACTIVATION EPITOPE).

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:20 - 1:200), IP, WB

Usage: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS

Preservative: Azide free

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: ITGB2 / MAC-1 / CD18

Background: Name/Gene ID: ITGB2
Family: Integrin

Synonyms: ITGB2, CD18 antigen, CD18, Integrin beta chain, beta 2, LCAMB, MAC-1, MFI7, MF17, Integrin beta-2, LAD, LFA-1

Gene ID: 3689

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation