STAT5B antibody (AA 774-787)

Catalog No. ABIN210645

The Rabbit Polyclonal anti-STAT5B antibody (ABIN210645) specifically detects STAT5B in WB and IP. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Horse, Pig and Hamster samples.

Quick Overview for STAT5B antibody (AA 774-787) (ABIN210645)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-STAT5B Antibody

Binding Specificity: AA 774-787

Specificity: Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%) Panda, Bat, Chicken, Xenopus (93%) Opossum (86%).

Purification: Protein A purified

Immunogen: Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%), Panda, Bat, Chicken, Xenopus (93%), Opossum (86%).

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Western Blot: 0.5-2 mg/mL detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 μg immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 μg protein) was incubated with 4 μg of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 μL of 2-mercaptoethanol. per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Alternative Name: STAT5B / STAT

Background: Name/Gene ID: STAT5B

Synonyms: STAT5B, Transcription factor STAT5B, STAT5

Gene ID: 6777

UniProt: P51692

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation