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FAK antibody (AA 748-1053)

The Rabbit Polyclonal anti-FAK antibody is suitable to detect FAK in samples from Human, Mouse, Rat and Hamster. It has been validated for WB, IP and ICC.
Catalog No. ABIN210881
$1,360.33
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 15 Business Days

Quick Overview for FAK antibody (AA 748-1053) (ABIN210881)

Target

See all FAK (PTK2) Antibodies
FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity

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Human, Mouse, Rat, Hamster

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This FAK antibody is un-conjugated

Application

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Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
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    AA 748-1053

    Specificity

    Recognizes human p125 FAK , does not cross-react with Pyk2. Species cross-reactivity: mouse, rat and hamster not avian.

    Purification

    Protein A purified

    Immunogen

    PGEX-derived fusion protein containing aa748-1053 of human FAK.

    Type of Immunogen: Fusion protein

    Isotype

    IgG
  • Application Notes

    Approved: ICC, IP, WB (0.5 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates FAK from 500 μg of murine 3T3 RIPA lysate. Positive control: F4209: Fibroblast Cell, 3T3/A31, use 20 μg per lane for minigels.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Target

    FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

    Alternative Name

    FAK / Focal Adhesion Kinase

    Background

    Name/Gene ID: PTK2
    Subfamily: Focal Adhesion Kinase
    Family: Protein Kinase

    Synonyms: PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK

    Gene ID

    5747

    UniProt

    Q05397

    Pathways

    Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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