GRIK1 antibody (AA 937-949)

Catalog No. ABIN210888

Product Details anti-GRIK1 Antibody

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Binding Specificity: AA 937-949

Reactivity: Human, Rat, Mouse, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes GluR5 isoforms, Mr 105-110kD. Species cross-reactivity: Rat. Predicted to cross-react with certain human and mouse GluR5 isoforms, based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Rabbit (100%) Bovine, Horse (92%) Chicken, Lizard (85%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 937-949 (CHQRRTQRKETVA) of rat GluR5-2C isoform precursor. This sequence is identical in rat isoforms GluR5-2 and GluR5-2B/GluR5-1 and human GluR5 isoform 2/EAA3A, and is not present in rat isoform GluR5-2A and human GluR5 isoform 1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Rabbit (100%), Bovine, Horse (92%), Chicken, Lizard (85%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Recommended Dilution: Western Blot: 0.5-2 μg/mL detects GluR5 in RIPA lysates from a rat brain microsomal preparation. Western Blot Analysis: Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-GluR5 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Alternative Name: GRIK1 / GLUR5 (GRIK1 Products)

Background: Name/Gene ID: GRIK1
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIK1, GluR-5, GLUR5, EEA3, GluK1, Glutamate receptor 5, EAA3, GLR5

Gene ID: 2897

UniProt: P39086

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity