p21 antibody

Catalog No. ABIN210984

The Mouse Monoclonal anti-p21 antibody (ABIN210984) specifically detects p21 in WB and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for p21 antibody (ABIN210984)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-p21 Antibody

Specificity: Recognizes WAF1, p21,~21kD. Species cross-reactivity: mouse.

Purification: Protein G purified

Immunogen: Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag.

Type of Immunogen: Recombinant protein

Isotype: IgG1, IgG2b kappa

Application Details

Application Notes: Approved: IP, WB

Usage: Suitable for use in Immunoprecipitation, Western Blot. Immunoprecipitation: 4 μg immunoprecipitates Cip1 from 500 μg of HeLa nuclear extract. Western Blot Analysis: 0.5-2 μg/mL detects WAF1, p21 in 20 μg of HeLa nuclear extract. HeLa nuclear extract was resolved by SDS PAGE, transferred to nitrocellulose, and probed with anti-WAF1, p21 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: HeLa nuclear extract. Use 20 μg per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.5 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A / WAF1 / p21

Background: Name/Gene ID: CDKN1A

Synonyms: CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21

Gene ID: 1026

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy