CDK5 antibody

Catalog No. ABIN211124

The Mouse Monoclonal anti-CDK5 antibody has been validated for WB, ELISA, IP and ICC. It is suitable to detect CDK5 in samples from Human, Mouse, Rat and Drosophila melanogaster.

Quick Overview for CDK5 antibody (ABIN211124)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human, Mouse, Rat, Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CDK5 Antibody

Specificity: Recognizes human cdk5, Mr 31kD. Species cross-reactivity: mouse, rat and Drosophila.

Purification: Protein G purified

Immunogen: Recombinant human cdk5.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC (5 μg/mL), IP, WB

Usage: Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and ELISA. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots of antibody detected cdk5 in RIPA lysates from mouse Wehi cells and rat PC12 cells. Immunoprecipitation: 4 μg/500 μg of A431 cell lysate. Immunocytochemistry: 5 μg/mL stains cdk5 in human A431 cells fixed with 4 % paraformaldehyde.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background: Name/Gene ID: CDK5
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK5, CDKN5, Cell division protein kinase 5, Crk6, Cyclin-dependent kinase 5, Protein kinase CDK5 splicing, PSSALRE, TPKII catalytic subunit

Gene ID: 1020

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis