CBL antibody (AA 878-906)
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- Target See all CBL Antibodies
- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
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Binding Specificity
- AA 878-906
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Reactivity
- Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CBL antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- Specific for Cbl. Species cross-reactivity: mouse and rat.
- Purification
- Protein G purified
- Immunogen
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GST fusion protein corresponding to residues 878-906 of human Cbl. This sequence is identical in mouse.
Type of Immunogen: Fusion protein - Isotype
- IgG
- Top Product
- Discover our top product CBL Primary Antibody
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- Application Notes
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Approved: ICC (5 - 10 μg/mL), IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot Analysis: 0.5-2 μg/mL detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cbl (1 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2 μg of a previous lot immunoprecipitated Cbl from 1 μg of a mouse 3T3 cell RIPA lysate. Immunocytochemistry: 5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute. Positive control: F4209:3T3 lysate. Use 20 μg per lane for minigels. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 20 % glycerol, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
- Alternative Name
- c-CBL (CBL Products)
- Synonyms
- 4732447J05Rik antibody, Cbl-2 antibody, c-Cbl antibody, cbl antibody, C-CBL antibody, CBL2 antibody, FRA11B antibody, NSLL antibody, RNF55 antibody, c-cbl-like antibody, RGD1561386 antibody, Casitas B-lineage lymphoma antibody, Cbl proto-oncogene antibody, Cbl antibody, CBL antibody
- Background
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Name/Gene ID: CBL
Synonyms: CBL, C-CBL, Oncogene CBL2, RING finger protein 55, Proto-oncogene c-Cbl, RNF55, CBL2, FRA11B, NSLL - Gene ID
- 867
- UniProt
- P22681
- Pathways
- TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals
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