CD47 antibody

Catalog No. ABIN7470769

The Mouse Monoclonal anti-CD47 antibody has been validated for FACS, WB, IHC, IP and IHC (fro). It is suitable to detect CD47 in samples from Human, Pig, Cow, Dog and Sheep.

Quick Overview for CD47 antibody (ABIN7470769)

Target: CD47

Reactivity: Human, Pig, Cow, Dog, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BRIC126

Product Details anti-CD47 Antibody

Specificity: Recognizes the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.

Purification: Protein G purified

Immunogen: Human erythrocytes.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (1:10), IHC, IHC-Fr, IP, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.BRIC126 recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction. 

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: TBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: Name/Gene ID: CD47

Synonyms: CD47, CD47 antigen, CD47 Molecule, Integrin associated protein, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6, Rh-related antigen, OA3, CD47 glycoprotein, IAP

Gene ID: 961