LCK antibody (AA 1-58)

Catalog No. ABIN211979

The Rabbit Polyclonal anti-LCK antibody is suitable to detect LCK in samples from Human, Mouse and Cow. It has been validated for WB and IP.

Quick Overview for LCK antibody (AA 1-58) (ABIN211979)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-LCK Antibody

Binding Specificity: AA 1-58

Specificity: Recognizes Lck, Mr 56kD of mouse, human and bovine, reactivity with other Src family members not determined.

Purification: Protein A purified

Immunogen: GST fusion protein corresponding to amino acids 1-58 of human Lck.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Western Blot Analysis: 0.5-2 μg/mL detected Lck in RIPA lysates from Jurkat cells. Included Positive Antigen Control: Jurkat cell lysate. Use 20 μg per lane for minigels. Immunoprecipitation: 4 μg immunoprecipitated Lck from 1 μg of Jurkat RIPA lysate as confirmed by subsequent immunoblot using monoclonal anti-Lck. Western Blot Analysis Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Lck (1 μg/mL). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: p56lck / LCK

Background: Name/Gene ID: LCK
Subfamily: Src
Family: Protein Kinase

Synonyms: LCK, Lck kinase, LSK, p56-LCK, Pp58lck, Tyrosine-protein kinase Lck, p56lck, Protein YT16, Proto-oncogene Lck, YT16

Gene ID: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling