GPR124 antibody (N-Term)

Catalog No. ABIN213374

Product Details anti-GPR124 Antibody

Target: GPR124 (G Protein-Coupled Receptor 124 (GPR124))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR124 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GPR124. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SEMA4G (55 % ), CSF3 (40 %).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (90%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human GPR124. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (90%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (11 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 11 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR124

Target: GPR124 (G Protein-Coupled Receptor 124 (GPR124))

Alternative Name: ADGRA2 / GPR124 (GPR124 Products)

Synonyms: TEM5 antibody, 8430414O08Rik antibody, 9530074E10Rik antibody, Tem5 antibody, mKIAA1531 antibody, MGC69043 antibody, si:dkey-208c22.2 antibody, GPR124 antibody, adhesion G protein-coupled receptor A2 antibody, adhesion G protein-coupled receptor A2 L homeolog antibody, G-protein coupled receptor 124 antibody, ADGRA2 antibody, Adgra2 antibody, adgra2.L antibody, adgra2 antibody, LOC616111 antibody

Background: Name/Gene ID: ADGRA2
Subfamily: Orphan-U
Family: GPCR

Synonyms: ADGRA2, G protein-coupled receptor 124, G-protein coupled receptor 124, TEM5, GPR124, KIAA1531, Tumor endothelial marker 5

Gene ID: 25960