SAFB antibody (AA 345-357)

Catalog No. ABIN214260

Product Details anti-SAFB Antibody

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Binding Specificity: AA 345-357

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAFB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%).

Purification: Protein G purified from tissue culture supernatant

Immunogen: Synthetic peptide from human SAFB. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%).

Type of Immunogen: Synthetic peptide

Clone: 6F7

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. Sourced in TCS.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SAFB

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Alternative Name: SAFB1 / SAFB (SAFB Products)

Synonyms: HAP antibody, HET antibody, SAF-B1 antibody, SAFB1 antibody, 3110021E02Rik antibody, 5330423C17Rik antibody, AU018122 antibody, D18386 antibody, E130307D12 antibody, zgc:55387 antibody, wu:fb40a03 antibody, wu:fd42g06 antibody, SAFB2 antibody, SAFB antibody, scaffold attachment factor B antibody, scaffold attachment factor B S homeolog antibody, SAFB-like transcription modulator antibody, Scaffold attachment factor B antibody, SAFB antibody, Safb antibody, safb antibody, safb.S antibody, LOC5570070 antibody, CpipJ_CPIJ017179 antibody, Bm1_49330 antibody, LOAG_00080 antibody

Background: Name/Gene ID: SAFB

Synonyms: SAFB, HAP, HET, Scaffold attachment factor B, SAB-B1, SAFB1, SAF-B, SAF-B1, Scaffold attachment factor B1, Hsp27 ERE-TATA binding protein, HSP27 ERE-TATA-binding protein

Gene ID: 6294

UniProt: Q15424

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway