IGF1 antibody

Catalog No. ABIN289466

The Rabbit Polyclonal anti-IGF1 antibody has been validated for WB, ELISA and Inhibition. It is suitable to detect IGF1 in samples from Human.

Quick Overview for IGF1 antibody (ABIN289466)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Product Details anti-IGF1 Antibody

Purification: Purified

Immunogen: IGF1 antibody was raised in rabbit using highly pure recombinant human IGF-I as the immunogen.

Application Details

Application Notes: ELISA: 0.5-2 μg/mL, Inhibition: 0.18-0.28 μg/mL, WB: 0.1-0.2 μg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1

Background: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.

Molecular Weight: 7.6 kDa (predicted detection band MW)

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation