HMGN1 antibody (pSer20, Ser24)

Catalog No. ABIN214463

The Rabbit Polyclonal anti-HMGN1 antibody has been validated for WB, IHC, ELISA and IHC (p). It is suitable to detect HMGN1 in samples from Human.

Quick Overview for HMGN1 antibody (pSer20, Ser24) (ABIN214463)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HMGN1 Antibody

Binding Specificity: pSer20, Ser24

Specificity: Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human HMGN1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:25000), IHC, IHC-P (20 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Alternative Name: HMGN1

Background: Name/Gene ID: HMGN1

Synonyms: HMGN1, HMG14

Gene ID: 3150

UniProt: P05114

Pathways: Chromatin Binding