PPARG antibody (Internal Region)

Catalog No. ABIN214562

The Rabbit Polyclonal anti-PPARG antibody is suitable to detect PPARG in samples from Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Pig, Hamster, Duck, Mink, Macaque and Squirrel. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for PPARG antibody (Internal Region) (ABIN214562)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Pig, Hamster, Duck, Mink, Macaque, Squirrel

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPARG Antibody

Binding Specificity: Internal Region

Specificity: Amino acids 255 -268 of human PPAR gamma isoform 1.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human PPARG.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:8000 - 1:32000), IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARG / PPAR Gamma

Background: Name/Gene ID: PPARG
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma

Gene ID: 5468

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation