BARD1 antibody (AA 108-124)

Catalog No. ABIN214634

The Rabbit Polyclonal anti-BARD1 antibody is suitable to detect BARD1 in samples from Human. It has been validated for ELISA, IHC and IHC (p).

Quick Overview for BARD1 antibody (AA 108-124) (ABIN214634)

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BARD1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BARD1 Antibody

Binding Specificity: AA 108-124

Specificity: Amino acids 108 to 124 of human BARD1

Purification: Protein G purified

Immunogen: Synthetic peptide from human BARD1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:1000), IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BARD1

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Alternative Name: BARD1

Background: Name/Gene ID: BARD1

Synonyms: BARD1, BARD-1, BRCA1 associated RING domain 1

Gene ID: 580

UniProt: Q99728

Pathways: DNA Damage Repair