ELISA: 0.5-2 μg/mL, WB: 0.1-0.2 μg/mL Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in sterile water to a concentration of > 0.2 mg/mL.
Concentration
Lot specific
Buffer
Lyophilized from 0.5 x PBS, pH 7.4.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
Frøyland, Pedersen, Kvissel, Almaas, Pharo, Skålhegg, Mollnes, Rootwelt: "Effect of acidosis on IL-8 and MCP-1 during hypoxia and reoxygenation in human NT2-N neurons." in: Brain research, Vol. 1113, Issue 1, pp. 64-73, (2006) (PubMed).
The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross reacts across species.