CDK4 antibody

Catalog No. ABIN215700

The Mouse Monoclonal anti-CDK4 antibody (Clone DCS-156) (ABIN215700) specifically detects CDK4 in WB, IHC, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CDK4 antibody (ABIN215700)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DCS-156

Product Details anti-CDK4 Antibody

Specificity: Recognizes human cdk4 protein present predominantly in G1 phase of cell cycle. It does not cross-react with cyclins. In immunohistochemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) the antibody shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.

Purification: Protein A purified

Immunogen: Human recombinant full-length cdk4 polypeptide.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Western Blot and Immunohistochemistry (frozen and paraffin-embedded tissue, improved after microwave treatment). Immunohistochemistry: 1:10, Incubation Time 1-4 h at RT or overnight at 2-8?C.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 0.09 % sodium azide, 0.5 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: Name/Gene ID: CDK4
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK4, Cell division protein kinase 4, Crk3, Cyclin-dependent kinase 4, CMM3, p34/cdk4, PSK-J3

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size