CDK4 antibody

Catalog No. ABIN215701

This Mouse Monoclonal antibody specifically detects CDK4 in WB, IF, IP and Func. It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for CDK4 antibody (ABIN215701)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func)

Clone: DCS-35

Product Details anti-CDK4 Antibody

Specificity: Recognizes a 34kD protein identified as p34 or cyclin-dependent kinase 4 (cdk4). It shows no cross-reaction with other cdks. Species cross-reactivity: Human, mouse, rat and porcine.

Purification: Protein G purified

Immunogen: Purified recombinant human cdk4 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Func, IF, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunofluorescence,. Immunoprecipitation and Kinase Assay. Western Blot: 1-2 μg/mL for 2 hrs at RT. Immunoprecipitation: Excellent for coprecipitation of D-type cyclins bound to cdk4.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: Name/Gene ID: CDK4
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK4, Cell division protein kinase 4, Crk3, Cyclin-dependent kinase 4, CMM3, p34/cdk4, PSK-J3

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size